验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, fetal human brain tissue, human testis tissue, rat brain tissue |
| Positive IHC detected in | human lung cancer tissue, human oesophagus cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy MAGEA3 Ready-To-Use IHC Kit. MAGEA3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
60054-1-Ig targets MAGEA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human |
| 免疫原 | MAGEA3 fusion protein Ag0265 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | melanoma antigen family A, 3 |
| 别名 | Antigen MZ2 D, Cancer/testis antigen 1.3, CT1.3, HIP8, HYPD, MAGE 3 antigen, MAGE3, MAGEA3, MAGE-A3, MAGEA6, melanoma antigen family A, 3, Melanoma associated antigen 3 |
| 计算分子量 | 314 aa, 35 kDa |
| 观测分子量 | 45-48 kDa |
| GenBank蛋白编号 | BC000340 |
| 基因名称 | MAGEA3 |
| Gene ID (NCBI) | 4102 |
| RRID | AB_2138124 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P43357 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAGEA3 is a member of the MAGEA family, , which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAGEA3 antibody 60054-1-Ig | Download protocol |
| IHC protocol for MAGEA3 antibody 60054-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS One MAGE-A3 is a prognostic biomarker for poor clinical outcome in cutaneous squamous cell carcinoma with perineural invasion via modulation of cell proliferation. | ||
J Transl Med Immunization with a multi-antigen targeted DNA vaccine eliminates chemoresistant pancreatic cancer by disrupting tumor-stromal cell crosstalk |
