• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

RABEP2 Monoclonal antibody

RABEP2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A4A4

Cat no : 60125-1-Ig

Print datasheet

Synonyms

FRA, Rabaptin 5beta, RABEP2, RABPT5B

验证数据展示

  • WB analysis of Jurkat using 60125-1-Ig

    WB analysis of Jurkat using 60125-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 60125-1-Ig

    WB analysis of HSC-T6 using 60125-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 60125-1-Ig

    IHC staining of human prostate cancer using 60125-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60125-1-Ig (RABEP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 60125-1-Ig

    IHC staining of human prostate cancer using 60125-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60125-1-Ig (RABEP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHSC-T6 cells, Jurkat cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60125-1-Ig targets RABEP2 in WB, IHC, ELISA applications and shows reactivity with human, rat, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, rat, pig
Immunogen RABEP2 fusion protein Ag6197 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name rabaptin, RAB GTPase binding effector protein 2
Synonyms FRA, Rabaptin 5beta, RABEP2, RABPT5B
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC058900
Gene Symbol RABEP2
Gene ID (NCBI) 79874
RRIDAB_2284821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H5N1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RABEP2, also named as RABPT5B, plays a role in membrane trafficking and in homotypic early endosome fusion. It has two isoforms with MW 59-63 kDa.

实验方案

Product Specific Protocols
WB protocol for RABEP2 antibody 60125-1-IgDownload protocol
IHC protocol for RABEP2 antibody 60125-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols