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验证数据展示

  • WB analysis of Raji using 60138-1-Ig

    WB analysis of Raji using 60138-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 60138-1-Ig (IL15RA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IL-15RA Monoclonal antibody

IL-15RA Monoclonal Antibody for WB, ELISA
Cat No. 60138-1-Ig

产品说明书

CloneNo. 5E3E1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, ELISA

别名

IL 15 receptor subunit alpha, IL 15R alpha, IL 15RA, IL15RA, IL-15RA, interleukin 15 receptor, alpha, sIL 15 receptor subunit alpha, sIL 15R alpha, sIL 15RA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, Daudi cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60138-1-Ig targets IL-15RA in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 IL-15RA fusion protein Ag10200 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 15 receptor, alpha
别名 IL 15 receptor subunit alpha, IL 15R alpha, IL 15RA, IL15RA, IL-15RA, interleukin 15 receptor, alpha, sIL 15 receptor subunit alpha, sIL 15R alpha, sIL 15RA
计算分子量 231 aa, 24 kDa
观测分子量 55 kDa
GenBank蛋白编号BC107777
基因名称 IL-15RA
Gene ID (NCBI) 3601
RRIDAB_2881352
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13261
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897). The calculated molecular weight of full-length IL-15Rα is 28 kDa, IL-15Rα can be glycosylated, and larger apparent molecular weights of 55-65 kDa have been reported by some researches (PMID: 10480910; 15976182; 15671076; 17912462).

实验方案

Product Specific Protocols
WB protocol for IL-15RA antibody 60138-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols