CXCL8/IL-8 Monoclonal antibody, PBS Only (Detector)
CXCL8/IL-8 Monoclonal Antibody for Sandwich ELISA, Indirect ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
Sandwich ELISA, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
3D11D5
验证数据展示
Planning an IHC experiment? We recommend our IHCeasy CXCL8/IL-8 Ready-To-Use IHC Kit. CXCL8/IL-8 primary antibody included.
产品信息
60141-2-PBS targets CXCL8/IL-8 as part of a matched antibody pair:
MP50089-1: 68744-1-PBS capture and 60141-2-PBS detection (validated in Sandwich ELISA)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Applications | Sandwich ELISA, Indirect ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | CXCL8/IL-8 fusion protein Ag10552 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | interleukin 8 |
| Synonyms | C X C motif chemokine 8, CXCL8, CXCL8/IL-8, Emoctakin, GCP 1, GCP1, IL8, IL-8, interleukin 8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, Neutrophil activating factor, T cell chemotactic factor |
| Calculated Molecular Weight | 99 aa, 11 kDa |
| GenBank Accession Number | BC013615 |
| Gene Symbol | IL8 |
| Gene ID (NCBI) | 3576 |
| ENSEMBL Gene ID | ENSG00000169429 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P10145 |
| Storage Buffer | PBS only |
| Storage Conditions | Store at -80°C. |
背景介绍
Interleukin 8 (IL-8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression. The human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa).