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验证数据展示

  • WB analysis of SGC-7901 using 60156-1-Ig

    WB analysis of SGC-7901 using 60156-1-Ig

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60156-1-Ig (AMOT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse skeletal muscle using 60156-1-Ig

    IF Staining of mouse skeletal muscle using 60156-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using AMOT antibody (60156-1-Ig, Clone: 3H7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  • KD/KO验证

AMOT Monoclonal antibody

AMOT Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 60156-1-Ig

产品说明书

CloneNo. 3H7B3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IF-P, ELISA

别名

KIAA1071, Angiomotin, 3H7B3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSGC-7901 cells, HEK-293 cells
Positive IF-P detected inmouse skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60156-1-Ig targets AMOT in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 peptide 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 angiomotin
别名 KIAA1071, Angiomotin, 3H7B3
计算分子量 118 kDa
观测分子量 130 kDa
GenBank蛋白编号NM_001113490
基因名称 AMOT
Gene ID (NCBI) 154796
RRIDAB_10644279
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ4VCS5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. The most abundant expression was found in the placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). This antibody only recognizes p130.

实验方案

Product Specific Protocols
WB protocol for AMOT antibody 60156-1-IgDownload protocol
IF protocol for AMOT antibody 60156-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols