RXRA Monoclonal antibody

RXRA Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4H6C4

Cat no : 60198-1-Ig

Print datasheet

Synonyms

NR2B1, Retinoid X receptor alpha, retinoid X receptor, alpha, RXRA, RXRα



经过测试的应用

Positive WB detected inHela cells
Positive IHC detected inhuman stomach cancer tissue, mouse cerebellum tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RXRA Ready-To-Use IHC Kit. RXRA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

60198-1-Ig targets RXRA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RXRA fusion protein Ag0987 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name retinoid X receptor, alpha
Synonyms NR2B1, Retinoid X receptor alpha, retinoid X receptor, alpha, RXRA, RXRα
Calculated Molecular Weight 462 aa, 51 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC007925
Gene Symbol RXRA
Gene ID (NCBI) 6256
RRIDAB_10896497
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19793
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high-affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of a ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation, and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in the liver, and also expressed in the lungs, kidneys, and heart. It can recognize the the mature 54 kDa RXRA and the truncated 44 kD RXRA (PMID: 20541701).

实验方案

Product Specific Protocols
WB protocol for RXRA antibody 60198-1-IgDownload protocol
IHC protocol for RXRA antibody 60198-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Endocrinol

Constitutive Androstane Receptor-Mediated Inhibition of Metformin on Phase II Metabolic Enzyme SULT2A1

Authors - Xiaowen Hu