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  • Featured Product
  • KD/KO Validated

ZAP70 Monoclonal antibody, PBS Only (Detector)

ZAP70 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A11G9

Cat no : 60200-1-PBS

Print datasheet

Synonyms

STD, SRK, EC:2.7.10.2, 70 kDa zeta-chain associated protein, 70 kDa zeta associated protein

验证数据展示

  • WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

    WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

    WB result of ZAP70 antibody (60200-1-Ig, 1:10000) with si-control and si-ZAP70 transfected Jurkat cells.. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

    IF Staining of human tonsillitis using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60200-1-Ig (ZAP70 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

    WB analysis of Jurkat using 60200-1-Ig (same clone as 60200-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60200-1-Ig (ZAP70 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

    IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

    IHC staining of human lymphoma using 60200-1-Ig (same clone as 60200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60200-1-Ig (ZAP70 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60200-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51134-1

    Cytometric bead array standard curve of MP51134-1

    Cytometric bead array standard curve of MP51134-1, ZAP70 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60200-3-PBS. Detection antibody: 60200-1-PBS. Standard:Ag7943. Range: 0.391-100 ng/mL.

产品信息

60200-1-PBS targets ZAP70 as part of a matched antibody pair:

MP51134-1: 60200-3-PBS capture and 60200-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, pig
Immunogen ZAP70 fusion protein Ag7943 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Synonyms STD, SRK, EC:2.7.10.2, 70 kDa zeta-chain associated protein, 70 kDa zeta associated protein
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC039039
Gene Symbol ZAP70
Gene ID (NCBI) 7535
ENSEMBL Gene IDENSG00000115085
RRIDAB_10944567
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43403
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.