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BCS1L Monoclonal antibody

BCS1L Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3G6H1

Cat no : 60212-1-Ig

Print datasheet

Synonyms

BCS1L, BJS, FLNMS, GRACILE, h BCS, h BCS1, Hs.6719, Mitochondrial chaperone BCS1, PTD

验证数据展示

  • WB analysis of HEK-293 using 60212-1-Ig

    WB analysis of HEK-293 using 60212-1-Ig

    WB result of BCS1L antibody (60212-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCS1L transfected HEK-293 cells.

  • WB analysis of COLO 320 using 60212-1-Ig

    WB analysis of COLO 320 using 60212-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 60212-1-Ig (BCS1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 60212-1-Ig

    IHC staining of human gliomas using 60212-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human gliomas using 60212-1-Ig

    IHC staining of human gliomas using 60212-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas using 60212-1-Ig(BCS1L antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of MCF-7 using 60212-1-Ig

    IF Staining of MCF-7 using 60212-1-Ig

    Immunofluorescent analysis of () fixed MCF-7 cells using 60212-1-Ig (BCS1L antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inColo320 cells, COLO 320 cells, HEK-293 cells
Positive IHC detected inhuman gliomas tissue, human brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60212-1-Ig targets BCS1L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen BCS1L fusion protein Ag18280 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name BCS1-like (yeast)
Synonyms BCS1L, BJS, FLNMS, GRACILE, h BCS, h BCS1, Hs.6719, Mitochondrial chaperone BCS1, PTD
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC007500
Gene Symbol BCS1L
Gene ID (NCBI) 617
RRIDAB_11042318
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y276
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human BCS1-like (BCS1L), a mitochondrial inner-membrane protein, is a chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Recently studies indicated that the mutations in this protein may cause the GRACILE (growth retardation, aminoaciduria, cholestasis, iron overload, lactacidosis, and early death) syndrome, a recessively inherited lethal disease characterized by fetal growth retardation, lactic acidosis, aminoaciduria, cholestasis, and abnormalities in iron metabolism.

实验方案

Product Specific Protocols
WB protocol for BCS1L antibody 60212-1-IgDownload protocol
IHC protocol for BCS1L antibody 60212-1-IgDownload protocol
IF protocol for BCS1L antibody 60212-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols