验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, rat brain tissue, mouse brain tissue, fetal human brain tissue |
| Positive IHC detected in | human cervical cancer tissue, human breast cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
60230-1-Ig targets FUT9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, pig, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | FUT9 fusion protein Ag8298 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | fucosyltransferase 9 (alpha (1,3) fucosyltransferase) |
| 别名 | Alpha (1,3) fucosyltransferase, Fuc TIX, Fucosyltransferase 9, Fucosyltransferase IX, FucT IX, FUT9 |
| 计算分子量 | 359 aa, 42 kDa |
| 观测分子量 | 44-46 kDa |
| GenBank蛋白编号 | BC036101 |
| 基因名称 | FUT9 |
| Gene ID (NCBI) | 10690 |
| RRID | AB_11183046 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9Y231 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FUT9 is the main enzyme responsible for the synthesis of Lewis X (Lex) and catalyzes the last step in the biosynthesis of Lewis X antigen by addition of a fucose residue to precursor glycan structures. FUT9 is expressed in stomach, kidney, brain, and in leukocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FUT9 antibody 60230-1-Ig | Download protocol |
| IHC protocol for FUT9 antibody 60230-1-Ig | Download protocol |
| IF protocol for FUT9 antibody 60230-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancers (Basel) FUT9-Driven Programming of Colon Cancer Cells towards a Stem Cell-Like State.
| ||
Acta Biochim Biophys Sin (Shanghai) ELF4 contributes to esophageal squamous cell carcinoma growth and metastasis by augmenting cancer stemness via FUT9
|
