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PLAA Monoclonal antibody

PLAA Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5C10G9

Cat no : 60244-1-Ig

Print datasheet

Synonyms

DOA1, PLA2P, PLAA, PLAP

验证数据展示

  • WB analysis of MCF-7 using 60244-1-Ig

    WB analysis of MCF-7 using 60244-1-Ig

    WB result of PLAA antibody (60244-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells.

  • WB analysis of ROS1728 using 60244-1-Ig

    WB analysis of ROS1728 using 60244-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60244-1-Ig (PLAA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 60244-1-Ig

    IHC staining of human kidney using 60244-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with PLAA antibody included
  • IHC staining of human kidney using 60244-1-Ig

    IHC staining of human kidney using 60244-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60244-1-Ig (PLAA Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with PLAA antibody included

经过测试的应用

Positive WB detected inROS1728 cells, K-562 cells, MCF-7 cells, A431 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PLAA Ready-To-Use IHC Kit. PLAA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 60244-1-Ig is PLAA Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PLAA fusion protein Ag3224 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phospholipase A2-activating protein
Synonyms DOA1, PLA2P, PLAA, PLAP
Calculated Molecular Weight 738 aa, 81 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC032551
Gene Symbol PLAA
Gene ID (NCBI) 9373
RRIDAB_2881366
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y263
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.

实验方案

Product Specific Protocols
WB protocol for PLAA antibody 60244-1-IgDownload protocol
IHC protocol for PLAA antibody 60244-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols