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CD14 Monoclonal antibody, PBS Only (Capture)

CD14 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C1D9

Cat No : 60253-1-PBS

Print datasheet

Synonyms

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 2C1D9

验证数据展示

  • WB analysis of THP-1 using 60253-1-Ig (same clone as 60253-1-PBS)

    WB analysis of THP-1 using 60253-1-Ig (same clone as 60253-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

    IF Staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD14 antibody (60253-1-Ig, Clone: 2C1D9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

    IHC staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig (CD14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD14 antibody included
  • IHC staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

    IHC staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig (CD14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD14 antibody included
  • IF Staining of RAW 264.7 using 60253-1-Ig (same clone as 60253-1-PBS)

    IF Staining of RAW 264.7 using 60253-1-Ig (same clone as 60253-1-PBS)

    Immunofluorescent analysis of RAW 264.7 cells using 60253-1-Ig (CD14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50282-2

    Cytometric bead array standard curve of MP50282-2

    Cytometric bead array standard curve of MP50282-2, CD14 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60253-1-PBS. Detection antibody: 60253-2-PBS. Standard:Ag10693. Range: 1.563-200 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.

产品信息

60253-1-PBS targets CD14 as part of a matched antibody pair:

MP50282-2: 60253-1-PBS capture and 60253-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen CD14 fusion protein Ag10693 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD14 molecule
Synonyms Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 2C1D9
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08571
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.