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CD39/ENTPD1 Monoclonal antibody

CD39/ENTPD1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D2H6

Cat No : 60323-1-Ig

Print datasheet

Synonyms

ATPDase, CD39, CD39/ENTPD1, Ecto apyrase, Ecto ATP diphosphohydrolase 1, Ecto ATPase 1, Ecto ATPDase 1, ENTPD1, e-NTPDase, NTPDase 1

验证数据展示

  • WB analysis using 60323-1-Ig

    WB analysis using 60323-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 60323-1-Ig

    IHC staining of human placenta using 60323-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 60323-1-Ig

    IHC staining of human placenta using 60323-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 60323-1-Ig

    IF Staining of HeLa using 60323-1-Ig

    Immunofluorescent analysis of HeLa cells using 60323-1-Ig (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60323-1-Ig targets CD39/ENTPD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CD39/ENTPD1 fusion protein Ag6165 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ectonucleoside triphosphate diphosphohydrolase 1
Synonyms ATPDase, CD39, CD39/ENTPD1, Ecto apyrase, Ecto ATP diphosphohydrolase 1, Ecto ATPase 1, Ecto ATPDase 1, ENTPD1, e-NTPDase, NTPDase 1
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC047664
Gene Symbol CD39
Gene ID (NCBI) 953
RRIDAB_2881434
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49961
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa, and 34 kDa) produced by alternative splicing. The soluble isoform of CD39(38-478 amino acid) is located in the extracellular part and 60323-1-Ig can recognize the soluble form of CD39 (38-478aa) molecule.

实验方案

Product Specific Protocols
WB protocol for CD39/ENTPD1 antibody 60323-1-IgDownload protocol
IHC protocol for CD39/ENTPD1 antibody 60323-1-IgDownload protocol
IF protocol for CD39/ENTPD1 antibody 60323-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols