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验证数据展示

  • IHC staining of human tonsillitis using 60349-1-Ig (same clone as 60349-1-PBS)

    IHC staining of human tonsillitis using 60349-1-Ig (same clone as 60349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60349-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60349-1-Ig (same clone as 60349-1-PBS)

    IHC staining of human tonsillitis using 60349-1-Ig (same clone as 60349-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60349-1-PBS in a different storage buffer formulation.

  • WB analysis of Ramos using 60349-1-Ig (same clone as 60349-1-PBS)

    WB analysis of Ramos using 60349-1-Ig (same clone as 60349-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60349-1-PBS in a different storage buffer formulation.

  • FC experiment of Ramos using 60349-1-Ig (same clone as 60349-1-PBS)

    FC experiment of Ramos using 60349-1-Ig (same clone as 60349-1-PBS)

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human BSAP,PAX5 (60349-1-Ig, Clone:2H2D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 60349-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50228-2

    Cytometric bead array standard curve of MP50228-2

    Cytometric bead array standard curve of MP50228-2, BSAP,PAX5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60349-1-PBS. Detection antibody: 60349-3-PBS. Standard:Ag16023. Range: 3.125-100 ng/mL.

  • Featured Product
  • KD/KO验证

BSAP,PAX5 Monoclonal antibody, PBS Only (Capture)

BSAP,PAX5 Monoclonal Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 60349-1-PBS

产品说明书

CloneNo. 2H2D6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

PAX5, PAX 5, Paired box protein Pax-5, Paired box protein Pax 5, paired box 5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60349-1-PBS targets BSAP,PAX5 as part of a matched antibody pair:

MP50228-2: 60349-1-PBS capture and 60349-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 BSAP,PAX5 fusion protein Ag16023 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 paired box 5
别名 PAX5, PAX 5, Paired box protein Pax-5, Paired box protein Pax 5, paired box 5
计算分子量 391 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC156927
基因名称 PAX5
Gene ID (NCBI) 5079
RRIDAB_2881458
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ02548
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members.