Cadherin-17 Monoclonal antibody
Cadherin-17 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
3B10A9
验证数据展示
经过测试的应用
| Positive WB detected in | human colon tissue, pig colon tissue |
| Positive IHC detected in | human colon cancer tissue, human colon tissue, human pancreas cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy CDH17 Ready-To-Use IHC Kit. CDH17 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
60351-1-Ig targets Cadherin-17 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples.
| Tested Applications | WB, IHC, IF-P, ELISA Application Description |
| Cited Applications | IHC |
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Immunogen | Cadherin-17 fusion protein Ag19488 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cadherin 17, LI cadherin (liver-intestine) |
| Synonyms | CDH17, HPT1, HPT 1, CDH16, Cadherin 17 |
| Calculated Molecular Weight | 832 aa, 92 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC113464 |
| Gene Symbol | Cadherin-17 |
| Gene ID (NCBI) | 1015 |
| RRID | AB_2881460 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q12864 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cadherin-17 antibody 60351-1-Ig | Download protocol |
| IHC protocol for Cadherin-17 antibody 60351-1-Ig | Download protocol |
| IF protocol for Cadherin-17 antibody 60351-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
