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验证数据展示

  • WB analysis of HepG2 using 60352-1-Ig

    WB analysis of HepG2 using 60352-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 60352-1-Ig

    IHC staining of human lung cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 60352-1-Ig

    IHC staining of human lung cancer using 60352-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60352-1-Ig (INHBA-Specific Antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Inhibin beta A-Specific Monoclonal antibody

Inhibin beta A-Specific Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60352-1-Ig

产品说明书

CloneNo. 1F8D8

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IHC, ELISA

别名

Activin beta A chain, EDF, FRP, INHBA, INHBA-Specific, Inhibin beta A chain, inhibin, beta A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, human testis tissue, L02 cells, pig liver tissue
Positive IHC detected inhuman lung cancer tissue, human oesophagus cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60352-1-Ig targets Inhibin beta A-Specific in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 inhibin, beta A
别名 Activin beta A chain, EDF, FRP, INHBA, INHBA-Specific, Inhibin beta A chain, inhibin, beta A
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号NM_002192
基因名称 Inhibin beta A
Gene ID (NCBI) 3624
RRIDAB_2881461
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP08476
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Inhibin βA(INHBA) is a ligand in the transforming growth factor β (TGF-β) superfamily. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.

实验方案

Product Specific Protocols
WB protocol for Inhibin beta A-Specific antibody 60352-1-IgDownload protocol
IHC protocol for Inhibin beta A-Specific antibody 60352-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols