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Conjugate
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EVI1 Monoclonal antibody

EVI1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A12A6

Cat no : 60413-1-Ig

Print datasheet

Synonyms

MDS1 EVI1, EVI-1, EVI 1, EC:2.1.1.367, AML1 EVI 1

验证数据展示

  • WB analysis of MKN-45 using 60413-1-Ig

    WB analysis of MKN-45 using 60413-1-Ig

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 60413-1-Ig (EVI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 60413-1-Ig

    WB analysis using 60413-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60413-1-Ig (EVI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMKN-45 cells, Calu-3 cells, MDA-MB-231 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy EVI1 Ready-To-Use IHC Kit. EVI1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60413-1-Ig targets EVI1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen EVI1 fusion protein Ag34143 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ecotropic viral integration site 1
Synonyms MDS1 EVI1, EVI-1, EVI 1, EC:2.1.1.367, AML1 EVI 1
Calculated Molecular Weight 138 kDa
Observed Molecular Weight140-150 kDa
GenBank Accession NumberNM_001105077
Gene Symbol EVI1
Gene ID (NCBI) 2122
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ03112
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EVI1 antibody 60413-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols