MED18 Monoclonal antibody
MED18 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2D10G8
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, U2OS cells, HSC-T6 cells, NIH/3T3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60467-1-Ig targets MED18 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | MED18 fusion protein Ag7495 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | mediator complex subunit 18 |
| Synonyms | |
| Calculated Molecular Weight | 24 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC002694 |
| Gene Symbol | MED18 |
| Gene ID (NCBI) | 54797 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BUE0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MED18, also named as Mediator of RNA polymerase II transcription subunit 18, is a 208 amino acid protein, which belongs to the Mediator complex subunit 18 family. MED18 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED18 as a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED18 is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MED18 antibody 60467-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |