MYB/c-Myb Monoclonal antibody
MYB/c-Myb Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2A12C2
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HL-60 cells, Jurkat cells, MOLT-4 cells, K-562 cells, 4T1 cells, RAW 264.7 cells, pig thymus tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60550-1-Ig targets MYB/c-Myb in WB, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | human, mouse, pig |
| Immunogen | MYB/c-Myb fusion protein Ag34185 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | v-myb myeloblastosis viral oncogene homolog (avian) |
| Synonyms | MYB, c-Myb, Transcriptional activator Myb, Proto-oncogene c-Myb, Proto oncogene c Myb |
| Calculated Molecular Weight | 640 aa, 72 kDa |
| Observed Molecular Weight | 40-50 kDa, 72-80 kDa |
| GenBank Accession Number | BC064955 |
| Gene Symbol | c-Myb |
| Gene ID (NCBI) | 4602 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P10242 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYB/c-Myb antibody 60550-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |