验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HL-60 cells, Jurkat cells, MOLT-4 cells, K-562 cells, 4T1 cells, RAW 264.7 cells, pig thymus tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60550-1-Ig targets MYB/c-Myb in WB, ELISA applications and shows reactivity with human, mouse, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, pig |
| 免疫原 | MYB/c-Myb fusion protein Ag34185 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | v-myb myeloblastosis viral oncogene homolog (avian) |
| 别名 | MYB, c-Myb, Transcriptional activator Myb, Proto-oncogene c-Myb, Proto oncogene c Myb |
| 计算分子量 | 640 aa, 72 kDa |
| 观测分子量 | 40-50 kDa, 72-80 kDa |
| GenBank蛋白编号 | BC064955 |
| 基因名称 | c-Myb |
| Gene ID (NCBI) | 4602 |
| RRID | AB_3670267 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P10242 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYB/c-Myb antibody 60550-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |