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IGF2BP2 Monoclonal antibody

IGF2BP2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E5H8

Cat no : 60647-1-Ig

Print datasheet

Synonyms

insulin like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, IGF-II mRNA-binding protein 2, IGF2 mRNA-binding protein 2

验证数据展示

  • WB analysis using 60647-1-Ig

    WB analysis using 60647-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60647-1-Ig (IGF2BP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 60647-1-Ig

    WB analysis using 60647-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60647-1-Ig (IGF2BP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, HT-1080 cells, A431 cells, HuH-7 cells
Planning an IHC experiment? We recommend our IHCeasy IGF2BP2 Ready-To-Use IHC Kit. IGF2BP2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60647-1-Ig targets IGF2BP2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen IGF2BP2 fusion protein Ag33681 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name insulin-like growth factor 2 mRNA binding protein 2
Synonyms insulin like growth factor 2 mRNA binding protein 2, IMP-2, IMP2, IGF-II mRNA-binding protein 2, IGF2 mRNA-binding protein 2
Calculated Molecular Weight65 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC021290
Gene Symbol IGF2BP2
Gene ID (NCBI) 10644
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6M1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2), ia a member of a family of mRNA binding protein, which can bind to several mammalian cells' mRNAs. Thus it may involve in the regulation of translation of mRNA. IGF2BP2's polymorphisms is risk factor for developing type 2 diabetes. This antibody raise against part of N-terminus of human IGF2BP2. IGF2BP2 has some isoforms with the MW of 54-66 kDa. Some literature has reported that multiple bands can be detected (PMID: 22427968, 36322753, 23069990).

实验方案

Product Specific Protocols
WB protocol for IGF2BP2 antibody 60647-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols