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Anti-Human ICAM-1/CD54 (15.2), PBS Only

ICAM-1/CD54 Monoclonal Antibody for FC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

15.2

Cat no : 65075-1-PBS

Print datasheet

Synonyms

CD54 (ICAM-1), CD54, ICAM-1, ICAM1, ICAM 1

验证数据展示

  • FC experiment of human PBMCs using 65075-1-Ig (same clone as 65075-1-PBS)

    FC experiment of human PBMCs using 65075-1-Ig (same clone as 65075-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD54 (ICAM-1) (65075-1-Ig, Clone:15.2) and APC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65075-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65075-1-Ig (same clone as 65075-1-PBS)

    FC experiment of human PBMCs using 65075-1-Ig (same clone as 65075-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD54 (ICAM-1) (65075-1-Ig, Clone:15.2) and APC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65075-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50085-1

    Sandwich ELISA standard curve of MP50085-1

    Sandwich ELISA standard curve of MP50085-1, ICAM-1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65075-1-PBS. Detection antibody: 68693-1-PBS. Standard: Eg0570. Range: 62.5-4000 pg/mL.

Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.

产品信息

65075-1-PBS targets ICAM-1/CD54 as part of a matched antibody pair:

MP50085-1: 65075-1-PBS capture and 68693-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications FC, Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Rheumatoid synovial cells and human monocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms CD54 (ICAM-1), CD54, ICAM-1, ICAM1, ICAM 1
Calculated Molecular Weight 90 kDa
GenBank Accession NumberBC015969
Gene Symbol ICAM-1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP05362
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).