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Anti-Human CD8 (UCHT4)

CD8 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

UCHT4

Cat no : 65204-1-Ig

Print datasheet

Synonyms

CD8, CD8A, CD8a molecule, Leu2, MAL, p32

验证数据展示

  • FC experiment of human PBMCs using 65204-1-Ig

    FC experiment of human PBMCs using 65204-1-Ig

    1X10^6 human PBMCs were surface co-stained with CL750 Anti-Human CD3 and 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and FITC-Donkey anti-Mouse IgG at dilution 1:1000, or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65204-1-Ig

    FC experiment of human PBMCs using 65204-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD8 (65204-1-Ig, Clone: UCHT4) and FITC-Donkey anti-Mouse IgG at dilution 1:1000, or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD8A Ready-To-Use IHC Kit. CD8A primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65204-1-Ig targets CD8 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Thymocytes and Sézary T cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CD8a molecule
Synonyms CD8, CD8A, CD8a molecule, Leu2, MAL, p32
Calculated Molecular Weight 235 aa, 26 kDa
GenBank Accession NumberBC025715
Gene Symbol CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
RRIDAB_2881486
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01732
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

实验方案

Product Specific Protocols
FC protocol for CD8 antibody 65204-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols