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验证数据展示

  • FC experiment of HeLa using 65519-1-MR

    FC experiment of HeLa using 65519-1-MR

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-MR, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of HeLa using 65519-1-MR

    FC experiment of HeLa using 65519-1-MR

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Anti-Human CD107a / LAMP1 (H4A3) Mouse IgG1 Recombinant Antibody

CD107a / LAMP1 Recombinant Antibody for FC (Intra)
Cat No. 65519-1-MR
CloneNo. H4A3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Synonyms

CD107a, LAMP1, LAMP-1, LAMP 1, H4A3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65519-1-MR targets CD107a / LAMP1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Human adherent peripheral blood cells 种属同源性预测
Host / Isotype Mouse / IgG1
Class Recombinant
Type Antibody
Full Name lysosomal-associated membrane protein 1
Synonyms CD107a, LAMP1, LAMP-1, LAMP 1, H4A3
Calculated Molecular Weight45 kDa
GenBank Accession NumberBC006345
Gene Symbol LAMP1
Gene ID (NCBI) 3916
ENSEMBL Gene IDENSG00000185896
RRIDAB_3670305
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

实验方案

Product Specific Protocols
FC protocol for CD107a / LAMP1 antibody 65519-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols