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验证数据展示

  • IHC staining of human breast cancer using 66040-2-Ig

    IHC staining of human breast cancer using 66040-2-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66040-2-Ig (FKBP52 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FKBP4/FKBP52 antibody included
  • IHC staining of human breast cancer using 66040-2-Ig

    IHC staining of human breast cancer using 66040-2-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66040-2-Ig (FKBP52 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FKBP4/FKBP52 antibody included
  • WB analysis using 66040-2-Ig

    WB analysis using 66040-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66040-2-Ig (FKBP52 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of human breast cancer using 66040-2-Ig

    IF Staining of human breast cancer using 66040-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using FKBP52 antibody (66040-2-Ig, Clone: 1B5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human breast cancer using 66040-2-Ig

    IF Staining of human breast cancer using 66040-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using FKBP52 antibody (66040-2-Ig, Clone: 1B5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

FKBP52 Monoclonal antibody

FKBP52 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66040-2-Ig

产品说明书

CloneNo. 1B5F1

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, IF-P, ELISA

别名

51 kDa FK506 binding protein, 52 kDa FK506 binding protein, 52 kDa FKBP, 59 kDa immunophilin, FK506 binding protein 4, FK506 binding protein 4, 59kDa, FKBP 4, FKBP 52, FKBP4, FKBP51, FKBP52, FKBP59, HBI, HSP binding immunophilin, Hsp56, Immunophilin FKBP52, p52, p59, PPIase, PPIase FKBP4, Rotamase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy FKBP4/FKBP52 Ready-To-Use IHC Kit. FKBP4/FKBP52 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66040-2-Ig targets FKBP52 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, rat
免疫原 FKBP52 fusion protein Ag18704 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 FK506 binding protein 4, 59kDa
别名 51 kDa FK506 binding protein, 52 kDa FK506 binding protein, 52 kDa FKBP, 59 kDa immunophilin, FK506 binding protein 4, FK506 binding protein 4, 59kDa, FKBP 4, FKBP 52, FKBP4, FKBP51, FKBP52, FKBP59, HBI, HSP binding immunophilin, Hsp56, Immunophilin FKBP52, p52, p59, PPIase, PPIase FKBP4, Rotamase
计算分子量 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC007924
基因名称 FKBP52
Gene ID (NCBI) 2288
RRIDAB_11043344
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ02790
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBP52 (FK506 binding protein 4) is a member of the immunophilin protein family that comprises intracellular protein effectors of immunosuppressive drugs, and it is known as a steroid receptor-associated protein. As a novel regulator of microtubule dynamics, FKBP52 is associated with the motor protein dynein and with the cytoskeleton during mitosis. FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, and plays a role in modulating toxicity of Abeta peptides. The protein can associate with the Tau function, and may help to decipher and modulate the events involved in Tau-induced neurodegeneration. In addition, FKBP52 is likely to play a role in growth and development of the male genitalia.

实验方案

Product Specific Protocols
WB protocol for FKBP52 antibody 66040-2-IgDownload protocol
IHC protocol for FKBP52 antibody 66040-2-IgDownload protocol
IF protocol for FKBP52 antibody 66040-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols