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验证数据展示

  • WB analysis of Jurkat using 66134-1-Ig

    WB analysis of Jurkat using 66134-1-Ig

    WB result of GOSR2/Membrin antibody (66134-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOSR2/Membrin transfected Jurkat cells.

  • WB analysis using 66134-1-Ig

    WB analysis using 66134-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66134-1-Ig (GOSR2/Membrin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66134-1-Ig

    IHC staining of human liver using 66134-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66134-1-Ig (GOSR2/Membrin antibody at dilution of 1:50.

  • IHC staining of human liver using 66134-1-Ig

    IHC staining of human liver using 66134-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66134-1-Ig (GOSR2/Membrin antibody at dilution of 1:50.

  • IF Staining of human liver cancer using 66134-1-Ig

    IF Staining of human liver cancer using 66134-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GOSR2/Membrin antibody (66134-1-Ig, Clone: 7E7C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

GOSR2/Membrin Monoclonal antibody

GOSR2/Membrin Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66134-1-Ig

产品说明书

CloneNo. 7E7C7
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

human, rat

应用

WB, IHC, IF-P, ELISA

别名

27 kDa Golgi SNARE protein, Bos1, GOSR2, GS27, Membrin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, Jurkat cells, PC-12 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66134-1-Ig targets GOSR2/Membrin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, rat
文献引用反应性human
免疫原 GOSR2/Membrin fusion protein Ag21393 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 golgi SNAP receptor complex member 2
别名 27 kDa Golgi SNARE protein, Bos1, GOSR2, GS27, Membrin
计算分子量 212 aa, 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC009710
基因名称 Membrin
Gene ID (NCBI) 9570
RRIDAB_2881533
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14653
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the solube NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in Golgi apparatus. It is involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.

实验方案

Product Specific Protocols
WB protocol for GOSR2/Membrin antibody 66134-1-IgDownload protocol
IHC protocol for GOSR2/Membrin antibody 66134-1-IgDownload protocol
IF protocol for GOSR2/Membrin antibody 66134-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Sci

Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification.

Authors - Dolma Choezom
View Article