LBP Monoclonal antibody
LBP Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human and More (1)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
3A7E3
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human liver cancer tissue, human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy LBP Ready-To-Use IHC Kit. LBP primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
66181-1-Ig targets LBP in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | LBP fusion protein Ag13485 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | lipopolysaccharide binding protein |
| Synonyms | LBP |
| Calculated Molecular Weight | 481 aa, 53 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC022256 |
| Gene Symbol | LBP |
| Gene ID (NCBI) | 3929 |
| RRID | AB_2881576 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P18428 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LBP antibody 66181-1-Ig | Download protocol |
| IHC protocol for LBP antibody 66181-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Lipopolysaccharide binding protein resists hepatic oxidative stress by regulating lipid droplet homeostasis |
