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  • Featured Product
  • KD/KO Validated

p38 MAPK Monoclonal antibody, PBS Only (Capture)

p38 MAPK Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1C2

Cat no : 66234-1-PBS

Print datasheet

Synonyms

MAPK14, CSPB1, CSBP2, CSBP1, CSBP

验证数据展示

  • WB analysis of HEK-293 using 66234-1-Ig (same clone as 66234-1-PBS)

    WB analysis of HEK-293 using 66234-1-Ig (same clone as 66234-1-PBS)

    WB result of p38 MAPK antibody (66234-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p38 MAPK transfected HEK-293 cells. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • WB analysis using 66234-1-Ig (same clone as 66234-1-PBS)

    WB analysis using 66234-1-Ig (same clone as 66234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • WB analysis of human heart using 66234-1-Ig (same clone as 66234-1-PBS)

    WB analysis of human heart using 66234-1-Ig (same clone as 66234-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

    IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with p38 MAPK antibody included
  • IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

    IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with p38 MAPK antibody included
  • WB analysis using 66234-1-Ig (same clone as 66234-1-PBS)

    WB analysis using 66234-1-Ig (same clone as 66234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50419-1

    Cytometric bead array standard curve of MP50419-1

    Cytometric bead array standard curve of MP50419-1, p38 MAPK Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66234-1-PBS. Detection antibody: 68965-1-PBS. Standard:Ag5797. Range: 0.195-200 ng/mL.

  • Cytometric bead array standard curve of MP50419-2

    Cytometric bead array standard curve of MP50419-2

    Cytometric bead array standard curve of MP50419-2, p38 MAPK Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66234-1-PBS. Detection antibody: 68966-1-PBS. Standard:Ag5797. Range: 1.563-200 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy p38 MAPK Ready-To-Use IHC Kit. p38 MAPK primary antibody included.

产品信息

66234-1-PBS targets p38 MAPK as part of a matched antibody pair:

MP50419-1: 66234-1-PBS capture and 68965-1-PBS detection (validated in Cytometric bead array)

MP50419-2: 66234-1-PBS capture and 68966-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen p38 MAPK fusion protein Ag5797 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name mitogen-activated protein kinase 14
Synonyms MAPK14, CSPB1, CSBP2, CSBP1, CSBP
Calculated Molecular Weight 360 aa, 41 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC031574
Gene Symbol p38 MAPK
Gene ID (NCBI) 1432
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16539
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.