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验证数据展示

  • WB analysis of MCF-7 using 66249-1-Ig

    WB analysis of MCF-7 using 66249-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66249-1-Ig (TRIM44 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 66249-1-Ig

    IF Staining of MCF-7 using 66249-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 66249-1-Ig(TRIM44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

TRIM44 Monoclonal antibody

TRIM44 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66249-1-Ig

产品说明书

CloneNo. 1B12H12
引用文献(3)

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IF/ICC, ELISA and More (1)

别名

DIPB, HSA249128, MC7, Protein DIPB, TRIM44, tripartite motif containing 44

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, human testis tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66249-1-Ig targets TRIM44 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原 TRIM44 fusion protein Ag18771 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 tripartite motif-containing 44
别名 DIPB, HSA249128, MC7, Protein DIPB, TRIM44, tripartite motif containing 44
计算分子量 344 aa, 38 kDa
观测分子量 50 kDa
GenBank蛋白编号BC024031
基因名称 TRIM44
Gene ID (NCBI) 54765
RRIDAB_2881637
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96DX7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM44 is one of the family member of TRIM protein, which contains an N-terminal ubiquitin hydrolase-type zinc-finger domain, followed by a coiled-coil domain, a zinc-finger B-box homology domain, and a second coiled-coil domain near the C-terminus. Members of the TRIM protein family are involved in various cellular processes, such as cell proliferation, oncogenesis and antiviral defense.

实验方案

Product Specific Protocols
WB protocol for TRIM44 antibody 66249-1-IgDownload protocol
IF protocol for TRIM44 antibody 66249-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Immunol

Inducible TAP1 Negatively Regulates the Antiviral Innate Immune Response by Targeting the TAK1 Complex.

Authors - Zhangchuan Xia
View Article
humanWB

J Immunol

NAC1 Potentiates Cellular Antiviral Signaling by Bridging MAVS and TBK1.

Authors - Zhangchuan Xia
View Article
humanIF

Genes Genomics

Overexpression of TRIM44 mediates the NF-κB pathway to promote the progression of ovarian cancer

Authors - Yang Yu
View Article