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CLTB Monoclonal antibody

CLTB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F9C2

Cat no : 66270-1-Ig

Print datasheet

Synonyms

Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB

验证数据展示

  • WB analysis of pig brain using 66270-1-Ig

    WB analysis of pig brain using 66270-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66270-1-Ig (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66270-1-Ig

    IHC staining of human liver using 66270-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with CLTB antibody included
  • IHC staining of human liver using 66270-1-Ig

    IHC staining of human liver using 66270-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with CLTB antibody included
  • IF Staining of HeLa using 66270-1-Ig

    IF Staining of HeLa using 66270-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66270-1-Ig(CLTB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inpig brain tissue
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CLTB Ready-To-Use IHC Kit. CLTB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66270-1-Ig targets CLTB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, pig
Cited Reactivityhuman
Immunogen CLTB fusion protein Ag24347 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name clathrin, light chain (Lcb)
Synonyms Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 32-38 kDa
GenBank Accession NumberBC006457
Gene Symbol CLTB
Gene ID (NCBI) 1212
RRIDAB_2881655
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09497
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

实验方案

Product Specific Protocols
WB protocol for CLTB antibody 66270-1-IgDownload protocol
IHC protocol for CLTB antibody 66270-1-IgDownload protocol
IF protocol for CLTB antibody 66270-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

Proteomic analysis of intracellular protein corona of nanoparticles elucidates nano-trafficking network and nano-bio interactions.

Authors - Mengmeng Qin
View Article