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验证数据展示

  • WB analysis using 66286-1-Ig (same clone as 66286-1-PBS)

    WB analysis using 66286-1-Ig (same clone as 66286-1-PBS)

    Various tissue lysates were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

  • WB analysis of human skeletal muscle using 66286-1-Ig (same clone as 66286-1-PBS)

    WB analysis of human skeletal muscle using 66286-1-Ig (same clone as 66286-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66286-1-Ig (same clone as 66286-1-PBS)

    IHC staining of human heart using 66286-1-Ig (same clone as 66286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL3 antibody included
  • IHC staining of human heart using 66286-1-Ig (same clone as 66286-1-PBS)

    IHC staining of human heart using 66286-1-Ig (same clone as 66286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL3 antibody included
  • Sandwich ELISA standard curve of MP50642-3

    Sandwich ELISA standard curve of MP50642-3

    Sandwich ELISA standard curve of MP50642-3, Human MYL3 Monoclonal Matched Antibody Pair - PBS only. 66286-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag24535. 66286-5-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP50642-3

    Sample test of MP50642-3

    The mean MYL3 concentration was determined to be 23.06 ng/mL in mouse heart tissue extract based on a 6.9 mg/mL extract load and 3.2 ng/mL in mouse skeletal muscle tissue extract based on a 1.7 mg/mL extract load.

  • Cytometric bead array standard curve of MP50642-3

    Cytometric bead array standard curve of MP50642-3

    Cytometric bead array standard curve of MP50642-3, MYL3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66286-1-PBS. Detection antibody: 66286-5-PBS. Standard:Ag24535. Range: 0.098-6.25 ng/mL.

MYL3 Monoclonal antibody, PBS Only (Capture)

MYL3 Monoclonal Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 66286-1-PBS

产品说明书

CloneNo. 2E4B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb, MLC1V

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MYL3 Ready-To-Use IHC Kit. MYL3 primary antibody included.

产品信息

66286-1-PBS targets MYL3 as part of a matched antibody pair:

MP50642-3: 66286-1-PBS capture and 66286-5-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat, pig
免疫原 MYL3 fusion protein Ag24535 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin, light chain 3, alkali; ventricular, skeletal, slow
别名 Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb, MLC1V
计算分子量 22 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC009790
基因名称 MYL3
Gene ID (NCBI) 4634
RRIDAB_2881669
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP08590
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)