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CGGBP1 Monoclonal antibody

CGGBP1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F5G9

Cat no : 66524-1-Ig

Print datasheet

Synonyms

20 kDa CGG binding protein, CGG binding protein 1, CGGBP, CGGBP1, p20 CGGBP, p20 CGGBP DNA binding protein

验证数据展示

  • WB analysis using 66524-1-Ig

    WB analysis using 66524-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66524-1-Ig (CGGBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inMCF-7 cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Planning an IHC experiment? We recommend our IHCeasy CGGBP1 Ready-To-Use IHC Kit. CGGBP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66524-1-Ig targets CGGBP1 in WB, ELISA applications and shows reactivity with Human, Rat, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Rat, mouse
Immunogen CGGBP1 fusion protein Ag1129 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CGG triplet repeat binding protein 1
Synonyms 20 kDa CGG binding protein, CGG binding protein 1, CGGBP, CGGBP1, p20 CGGBP, p20 CGGBP DNA binding protein
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC005222
Gene Symbol CGGBP1
Gene ID (NCBI) 8545
RRIDAB_2881887
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UFW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CGGBP1, also named as CGGBP, is a 20 kDa CGG-binding protein. It binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. CGGBP1 may play a role in regulating FMR1 promoter. It is a bona fide midbody protein required for normal abscission and mitosis in general.(PMID:20832400)

实验方案

Product Specific Protocols
WB protocol for CGGBP1 antibody 66524-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols