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COX4NB Monoclonal antibody

COX4NB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B8D3

Cat no : 66547-1-Ig

Print datasheet

Synonyms

C16orf2, C16orf4, COX4 neighbor, COX4AL, COX4NB, FAM158B, Neighbor of COX4, NOC4, Protein FAM158B

验证数据展示

  • WB analysis using 66547-1-Ig

    WB analysis using 66547-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66547-1-Ig (COX4NB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66547-1-Ig

    IHC staining of human liver using 66547-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66547-1-Ig (COX4NB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66547-1-Ig

    IF Staining of HeLa using 66547-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COX4NB antibody (66547-1-Ig, Clone: 2B8D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66547-1-Ig targets COX4NB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat
Cited Reactivitymouse
Immunogen COX4NB fusion protein Ag13776 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name COX4 neighbor
Synonyms C16orf2, C16orf4, COX4 neighbor, COX4AL, COX4NB, FAM158B, Neighbor of COX4, NOC4, Protein FAM158B
Calculated Molecular Weight 210 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC001472
Gene Symbol COX4NB
Gene ID (NCBI) 10328
RRIDAB_2881909
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43402
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COX4NB antibody 66547-1-IgDownload protocol
IHC protocol for COX4NB antibody 66547-1-IgDownload protocol
IF protocol for COX4NB antibody 66547-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

iScience

The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors

Authors - Angela L. Whittsette
View Article