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YME1L1 Monoclonal antibody

YME1L1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B7E4

Cat no : 66551-1-Ig

Print datasheet

Synonyms

FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1

验证数据展示

  • WB analysis of HeLa using 66551-1-Ig

    WB analysis of HeLa using 66551-1-Ig

    WB result of YME1L1 antibody (66551-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YME1L1 transfected HeLa cells.

  • WB analysis using 66551-1-Ig

    WB analysis using 66551-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66551-1-Ig (YME1L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human kidney using 66551-1-Ig

    IHC staining of human kidney using 66551-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66551-1-Ig (YME1L1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 66551-1-Ig

    IHC staining of human kidney using 66551-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66551-1-Ig (YME1L1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, Raji cells, THP-1 cells, HSC-T6 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66551-1-Ig targets YME1L1 in WB, IHC, ELISA applications and shows reactivity with Human, Rat, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Rat, mouse
Immunogen YME1L1 fusion protein Ag2069 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name YME1-like 1 (S. cerevisiae)
Synonyms FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1
Calculated Molecular Weight 716 aa, 80 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC023507
Gene Symbol YME1L1
Gene ID (NCBI) 10730
RRIDAB_2881913
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96TA2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).

实验方案

Product Specific Protocols
WB protocol for YME1L1 antibody 66551-1-IgDownload protocol
IHC protocol for YME1L1 antibody 66551-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols