• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Huntingtin Monoclonal antibody

Huntingtin Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F6C9

Cat no : 66553-1-Ig

Print datasheet

Synonyms

HD, HD protein, HTT, huntingtin, Huntington disease protein, IT15

验证数据展示

  • IHC staining of human cerebellum using 66553-1-Ig

    IHC staining of human cerebellum using 66553-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66553-1-Ig (Huntingtin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HTT antibody included
  • IHC staining of human cerebellum using 66553-1-Ig

    IHC staining of human cerebellum using 66553-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66553-1-Ig (Huntingtin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HTT antibody included

经过测试的应用

Positive IHC detected inhuman cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HTT Ready-To-Use IHC Kit. HTT primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66553-1-Ig targets Huntingtin in IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen Huntingtin fusion protein Ag25922 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name huntingtin
Synonyms HD, HD protein, HTT, huntingtin, Huntington disease protein, IT15
Calculated Molecular Weight 348 kDa
GenBank Accession NumberNM_002111
Gene Symbol Huntingtin
Gene ID (NCBI) 3064
RRIDAB_2881915
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Huntingtin antibody 66553-1-IgDownload protocol
FC protocol for Huntingtin antibody 66553-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols