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PLDN Monoclonal antibody

PLDN Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4F4C11

Cat no : 66558-1-Ig

Print datasheet

Synonyms

PA, PALLID, Pallid protein homolog, Pallidin, pallidin homolog (mouse), PLDN

验证数据展示

  • WB analysis using 66558-1-Ig

    WB analysis using 66558-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66558-1-Ig (PLDN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, ROS1728 cells, RAW 264.7 cells, NIH/3T3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66558-1-Ig targets PLDN in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen PLDN fusion protein Ag18095 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pallidin homolog (mouse)
Synonyms PA, PALLID, Pallid protein homolog, Pallidin, pallidin homolog (mouse), PLDN
Calculated Molecular Weight 23~24 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC004819
Gene Symbol PLDN
Gene ID (NCBI) 26258
RRIDAB_2881919
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UL45
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pallidin, also named as PLDN and PA, is involved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules. It may play a role in intracellular vesicle trafficking, particularly in the vesicle-docking and fusion process. Pallidin interacts with syntaxin-12 which is a t-SNARE protein that mediates vesicle docking and fusion.

实验方案

Product Specific Protocols
WB protocol for PLDN antibody 66558-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols