HYAL1 Monoclonal antibody
HYAL1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (2)
Applications
WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1B8B5
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, LNCaP cells, PC-3 cells, DU 145 cells, NCCIT cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
66560-1-Ig targets HYAL1 in WB, IF, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Immunogen | HYAL1 fusion protein Ag18183 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | hyaluronoglucosaminidase 1 |
| Synonyms | HYAL 1, HYAL1, Hyaluronidase 1, hyaluronoglucosaminidase 1, LuCa 1, LUCA1, Lung carcinoma protein 1, NAT6 |
| Calculated Molecular Weight | 435 aa, 48 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC035695 |
| Gene Symbol | HYAL1 |
| Gene ID (NCBI) | 3373 |
| RRID | AB_2881921 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q12794 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hyaluronic acid (HA) is a glycosaminoglycan that is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. It is also an integral structural component of cartilage and other tissues and acts as a lubricant in joints. Hyaluronidases are a family of enzymes that catalyse the degradation of HA. In humans, there are five functional hyaluronidases: HYAL1, HYAL2, HYAL3, HYAL4 and HYAL5 (also known as SPAM1 or PH-20); plus a pseudogene, HYAL6 (also known as HYALP1). HYAL-1 is present in many tissues and is predominantly found in the plasma and urine (PMID: 16600643). In addition to its function in normal cellular hyaluronan turnover, human HYAL-1 is implicated in cancer proliferation, angiogenesis, and inflammatory diseases (PMID: 17503783).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HYAL1 antibody 66560-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Immunother Cancer An oncolytic vaccinia virus encoding hyaluronidase reshapes the extracellular matrix to enhance cancer chemotherapy and immunotherapy | ||
Appl Biochem Biotechnol Kunzea Ericoides (Kanuka) Leaf Extracts Show Moisturisation, Antioxidant, and UV Protection Effects in HaCaT Cells and Anti-melanogenesis Effects in B16F10 Cells |