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验证数据展示

  • WB analysis using 66568-1-Ig

    WB analysis using 66568-1-Ig

    Various cells were subjected to SDS PAGE followed by western blot with 66568-1-Ig (FOXC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66568-1-Ig

    WB analysis of HEK-293 using 66568-1-Ig

    WB result of FOXC1 antibody (66568-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXC1 transfected HEK-293 cells.

  • Featured Product
  • KD/KO验证

FOXC1 Monoclonal antibody

FOXC1 Monoclonal Antibody for WB, ELISA
Cat No. 66568-1-Ig

产品说明书

CloneNo. 1F4E11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, ELISA

别名

FREAC 3, Forkhead-related transcription factor 3, Forkhead-related protein FKHL7, FKHL7, ARA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HEK293 cells, L02 cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:20000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66568-1-Ig targets FOXC1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 forkhead box C1
别名 FREAC 3, Forkhead-related transcription factor 3, Forkhead-related protein FKHL7, FKHL7, ARA
计算分子量 57 kDa
观测分子量 70-75 kDa
GenBank蛋白编号NM_001453
基因名称 FOXC1
Gene ID (NCBI) 2296
RRIDAB_2881929
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ12948
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXC1, also named as FKHL7 and FREAC3, binding of FREAC-3 and FREAC-4 to their cognate sites results in bending of the DNA at an angle of 80-90 degrees. Defects in FOXC1 are the cause of Axenfeld-Rieger syndrome type 3 (RIEG3). Defects in FOXC1 are the cause of iridogoniodysgenesis anomaly (IGDA). Defects in FOXC1 are a cause of Peters anomaly.

实验方案

Product Specific Protocols
WB protocol for FOXC1 antibody 66568-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols