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验证数据展示

  • WB analysis of HEK-293 using 66595-1-Ig

    WB analysis of HEK-293 using 66595-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66595-1-Ig (MRPL23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66595-1-Ig

    IHC staining of human breast cancer using 66595-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66595-1-Ig (MRPL23 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 66595-1-Ig

    IHC staining of human breast cancer using 66595-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66595-1-Ig (MRPL23 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 66595-1-Ig

    IF Staining of HEK-293 using 66595-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 66595-1-Ig (MRPL23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

MRPL23 Monoclonal antibody

MRPL23 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66595-1-Ig

产品说明书

CloneNo. 1G11C4

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

L23MRP, L23mt, MRP L23, MRPL23, Ribosomal protein L23 like, RPL23, RPL23L

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66595-1-Ig targets MRPL23 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 MRPL23 fusion protein Ag2220 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L23
别名 L23MRP, L23mt, MRP L23, MRPL23, Ribosomal protein L23 like, RPL23, RPL23L
计算分子量 153 aa, 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC027710
基因名称 MRPL23
Gene ID (NCBI) 6150
RRIDAB_2881955
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL23 antibody 66595-1-IgDownload protocol
IHC protocol for MRPL23 antibody 66595-1-IgDownload protocol
IF protocol for MRPL23 antibody 66595-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols