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验证数据展示

  • WB analysis using 66657-1-Ig (same clone as 66657-1-PBS)

    WB analysis using 66657-1-Ig (same clone as 66657-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66657-1-Ig (ETV5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66657-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50024-1

    Sandwich ELISA standard curve of MP50024-1

    Sandwich ELISA standard curve ofMP50024-1, ETV5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66657-1-PBS. Detection antibody: HRP-conjugated 66657-2-PBS. Standard: Ag3708. Range: 0.313-20 ng/m.

ETV5 Monoclonal antibody, PBS Only (Capture)

ETV5 Monoclonal Antibody for WB, Sandwich ELISA, Indirect ELISA
Cat No. 66657-1-PBS

产品说明书

CloneNo. 2A4A4

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, Sandwich ELISA, Indirect ELISA

别名

ERM, Ets related protein ERM, ETS translocation variant 5, ets variant 5, ETV5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ETV5 Ready-To-Use IHC Kit. ETV5 primary antibody included.

产品信息

66657-1-PBS targets ETV5 as part of a matched antibody pair:

MP50024-1: 66657-1-PBS capture and 66657-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 Human
免疫原 ETV5 fusion protein Ag3708 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ets variant 5
别名 ERM, Ets related protein ERM, ETS translocation variant 5, ets variant 5, ETV5
计算分子量 510 aa, 58 kDa
观测分子量 58-60 kDa
GenBank蛋白编号BC007333
基因名称 ETV5
Gene ID (NCBI) 2119
RRIDAB_2882014
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP41161
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ETS variant gene 5(ETV5) belongs to the ETS oncogene family that shares a conserved peptide, ETS domain, which mediates sequence-specific DNA binding. ETV5 is a transcription factor and is required for spermatogonial stem cell self-renewal. ETV5 can be negatively regulated by COP1, a tumor suppressor. Also it has a role in branching morphogenesis in the developing kidney