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  • Featured Product
  • KD/KO Validated

Cytokeratin 13 Monoclonal antibody, PBS Only (Capture)

Cytokeratin 13 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E10G2

Cat no : 66684-1-PBS

Print datasheet

Synonyms

KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13

验证数据展示

  • WB analysis of A431 using 66684-1-Ig (same clone as 66684-1-PBS)

    WB analysis of A431 using 66684-1-Ig (same clone as 66684-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66684-1-Ig (KRT13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT13 antibody included
  • IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (KRT13 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT13 antibody included
  • IF Staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    IF Staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human cervical cancer tissue using Cytokeratin 13 antibody (66684-1-Ig, Clone: 1E10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

  • WB analysis of A431 using 66684-1-Ig (same clone as 66684-1-PBS)

    WB analysis of A431 using 66684-1-Ig (same clone as 66684-1-PBS)

    WB result of Cytokeratin 13 antibody (66684-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 13 transfected A431 cells. This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    IHC staining of human cervical cancer using 66684-1-Ig (same clone as 66684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66684-1-Ig (Cytokeratin 13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66684-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT13 antibody included
  • Cytometric bead array standard curve of MP51141-1

    Cytometric bead array standard curve of MP51141-1

    Cytometric bead array standard curve of MP51141-1, Cytokeratin 13 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66684-1-PBS. Detection antibody: 66684-2-PBS. Standard:Ag0217. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP51141-3

    Cytometric bead array standard curve of MP51141-3

    Cytometric bead array standard curve of MP51141-3, Cytokeratin 13 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66684-1-PBS. Detection antibody: 66684-4-PBS. Standard:Ag0217. Range: 0.098-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy KRT13 Ready-To-Use IHC Kit. KRT13 primary antibody included.

产品信息

66684-1-PBS targets Cytokeratin 13 as part of a matched antibody pair:

MP51141-1: 66684-1-PBS capture and 66684-2-PBS detection (validated in Cytometric bead array)

MP51141-3: 66684-1-PBS capture and 66684-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen Cytokeratin 13 fusion protein Ag0217 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 13
Synonyms KRT13, Cytokeratin,Keratin, ck17, CK-13, CK13
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC002661
Gene Symbol Cytokeratin 13
Gene ID (NCBI) 3860
RRIDAB_2882038
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13646
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. The Keratin 13 (KRT13) gene encodes a type I acidic keratin which is expressed in the differentiated cells of non-cornified stratified squamous epithelia. This antibody can react with Cytokeratin 15.