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  • Featured Product
  • KD/KO Validated

OCIAD1 Monoclonal antibody, PBS Only (Capture)

OCIAD1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C10C3

Cat no : 66698-1-PBS

Print datasheet

Synonyms

Ovarian cancer immunoreactive antigen domain containing 1, OCIA domain-containing protein 1, OCIA domain containing 1, OCIA, ASRIJ

验证数据展示

  • WB analysis of HeLa using 66698-1-Ig (same clone as 66698-1-PBS)

    WB analysis of HeLa using 66698-1-Ig (same clone as 66698-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66698-1-Ig (OCIAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    IHC staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    IHC staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66698-1-Ig (OCIAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    IF Staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    IF Staining of human liver cancer using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66698-1-Ig (same clone as 66698-1-PBS)

    IF Staining of HeLa using 66698-1-Ig (same clone as 66698-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OCIAD1 antibody (66698-1-Ig, Clone: 1C10C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66698-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50614-1

    Cytometric bead array standard curve of MP50614-1

    Cytometric bead array standard curve of MP50614-1, OCIAD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66698-1-PBS. Detection antibody: 66698-2-PBS. Standard:Ag9977. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50614-2

    Cytometric bead array standard curve of MP50614-2

    Cytometric bead array standard curve of MP50614-2, OCIAD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66698-1-PBS. Detection antibody: 66698-3-PBS. Standard:Ag9977. Range: 0.098-100 ng/mL.

产品信息

66698-1-PBS targets OCIAD1 as part of a matched antibody pair:

MP50614-1: 66698-1-PBS capture and 66698-2-PBS detection (validated in Cytometric bead array)

MP50614-2: 66698-1-PBS capture and 66698-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen OCIAD1 fusion protein Ag9977 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name OCIA domain containing 1
Synonyms Ovarian cancer immunoreactive antigen domain containing 1, OCIA domain-containing protein 1, OCIA domain containing 1, OCIA, ASRIJ
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC003409
Gene Symbol OCIAD1
Gene ID (NCBI) 54940
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NX40
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OCIAD1 was first identified by immunoscreening of an ovarian carcinoma cDNA expression library with ascites fluid from ovarian cancer patients (PMID: 11162530). OCIAD1 has been reported as a key player in ovarian cancer cell adhesion, as well as a key player in generating ovarian cancer recurrence (PMID: 18328549; 20515946). In addition to its roles in cancer, OCIAD1 participates in maintaining stem cell potency by regulating the Jak/STAT pathway (PMID: 23972987). Several alternatively spliced forms of OCIAD1 gene have been identified. The longest form (1.4 kb) is predicted to encode for a 27.6 kDa protein of 245 amino acids. This antibody detects OCIAD1 with an apparent molecular weight of ~35 kDa as has been demonstrated by several researches (PMID: 27345969; 27345976).