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RHEB Monoclonal antibody

RHEB Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2F9H8

Cat no : 66714-1-Ig

Print datasheet

Synonyms

RHEB2, Ras homolog enriched in brain, GTP-binding protein Rheb, GTP binding protein Rheb, EC:3.6.5.-

验证数据展示

  • WB analysis of fetal human brain using 66714-1-Ig

    WB analysis of fetal human brain using 66714-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of human pancreas cancer using 66714-1-Ig

    IF Staining of human pancreas cancer using 66714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse pancreas using 66714-1-Ig

    IF Staining of mouse pancreas using 66714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human pancreas cancer using 66714-1-Ig

    IF Staining of human pancreas cancer using 66714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human skin cancer using 66714-1-Ig

    IHC staining of human skin cancer using 66714-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66714-1-Ig (RHEB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RHEB antibody included

经过测试的应用

Positive WB detected infetal human brain tissue, HeLa cells, DU 145 cells, mouse brain tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman pancreas cancer tissue, mouse pancreas tissue
Planning an IHC experiment? We recommend our IHCeasy RHEB Ready-To-Use IHC Kit. RHEB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66714-1-Ig targets RHEB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen RHEB fusion protein Ag8887 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Ras homolog enriched in brain
Synonyms RHEB2, Ras homolog enriched in brain, GTP-binding protein Rheb, GTP binding protein Rheb, EC:3.6.5.-
Calculated Molecular Weight 184 aa, 20 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC016155
Gene Symbol RHEB
Gene ID (NCBI) 6009
RRIDAB_2882065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15382
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.

实验方案

Product Specific Protocols
WB protocol for RHEB antibody 66714-1-IgDownload protocol
IHC protocol for RHEB antibody 66714-1-IgDownload protocol
IF protocol for RHEB antibody 66714-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols