STAT6 Monoclonal antibody
STAT6 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat and More (1)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2E5B8
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, K-562 cells |
Positive IHC detected in | human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
IF | See 1 publications below |
产品信息
66717-1-Ig targets STAT6 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | STAT6 fusion protein Ag0410 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | signal transducer and activator of transcription 6, interleukin-4 induced |
Synonyms | D12S1644, IL 4 STAT, STAT6, STAT6B, STAT6C |
Calculated Molecular Weight | 100 kDa |
Observed Molecular Weight | 95 kDa |
GenBank Accession Number | BC005823 |
Gene Symbol | STAT6 |
Gene ID (NCBI) | 6778 |
RRID | AB_2882068 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P42226 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for STAT6 antibody 66717-1-Ig | Download protocol |
IHC protocol for STAT6 antibody 66717-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Immunol PPARγ as an E3 Ubiquitin-Ligase Impedes Phosphate-Stat6 Stability and Promotes Prostaglandins E2-Mediated Inhibition of IgE Production in Asthma. | ||
J Cell Mol Med Fructose-1,6-bisphosphatase aggravates oxidative stress-induced apoptosis in asthma by suppressing the Nrf2 pathway. | ||
Immunol Invest Absence of TNF Leads to Alternative Activation in Peritoneal Macrophages in Experimental Listeria Monocytogenes Infection. | ||
J Invest Dermatol Benvitimod Inhibits IL-4- and IL-13-Induced Tight Junction Impairment by Activating AHR/ARNT Pathway and Inhibiting STAT6 Phosphorylation in Human Keratinocytes |