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  • Featured Product
  • KD/KO Validated

AURKA Monoclonal antibody, PBS Only (Detector)

AURKA Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG3

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F4B10

Cat no : 66757-1-PBS

Print datasheet

Synonyms

Aurora A, AURK, AURA, ARK-1, AIRK1

验证数据展示

  • IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

    IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AURKA antibody included
  • IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

    IHC staining of human breast cancer using 66757-1-Ig (same clone as 66757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66757-1-Ig (AURKA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AURKA antibody included
  • WB analysis of human testis using 66757-1-Ig (same clone as 66757-1-PBS)

    WB analysis of human testis using 66757-1-Ig (same clone as 66757-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66757-1-Ig (same clone as 66757-1-PBS)

    IF Staining of HeLa using 66757-1-Ig (same clone as 66757-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AURKA antibody (66757-1-Ig, Clone: 1F4B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • WB analysis using 66757-1-Ig (same clone as 66757-1-PBS)

    WB analysis using 66757-1-Ig (same clone as 66757-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66757-1-Ig (AURKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66757-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50265-1

    Cytometric bead array standard curve of MP50265-1

    Cytometric bead array standard curve of MP50265-1, AURKA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66757-2-PBS. Detection antibody: 66757-1-PBS. Standard:Ag20096. Range: 0.391-50 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy AURKA Ready-To-Use IHC Kit. AURKA primary antibody included.

产品信息

66757-1-PBS targets AURKA as part of a matched antibody pair:

MP50265-1: 66757-2-PBS capture and 66757-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity rat, mouse, human
Immunogen AURKA fusion protein Ag20096 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name aurora kinase A
Synonyms Aurora A, AURK, AURA, ARK-1, AIRK1
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46-52 kDa
GenBank Accession NumberBC002499
Gene Symbol AURKA
Gene ID (NCBI) 6790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14965
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).