验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, HEK-293 cells, HT-29 cells, Caco-2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66796-1-Ig targets CNKSR1 in WB, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human |
免疫原 | CNKSR1 fusion protein Ag27855 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | connector enhancer of kinase suppressor of Ras 1 |
别名 | CNK, CNK homolog protein 1, CNK1, CNKSR1, Connector enhancer of KSR 1, Connector enhancer of KSR like, hCNK1, KSR |
计算分子量 | 80 kDa |
观测分子量 | 90 kDa |
GenBank蛋白编号 | BC011604 |
基因名称 | CNKSR1 |
Gene ID (NCBI) | 10256 |
RRID | AB_2882140 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CNKSR1 is a protein containing several motifs involved in protein-protein interaction, including PDZ, PH (Pleckstrin homology), and SAM (sterile alpha motif) domains. The encoded protein acts as a scaffold component for receptor tyrosine kinase signaling and may mediate crosstalk between different signaling pathways. CNKSR1 is required in multiple receptor tyrosine kinase pathways where it appears to be a tyrosine phosphorylation targe.
实验方案
Product Specific Protocols | |
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WB protocol for CNKSR1 antibody 66796-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |