CEP250,C-NAP1 Monoclonal antibody
CEP250,C-NAP1 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (2)
Applications
WB, IF/ICC, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
2G12D12
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 2 publications below |
| IF | See 2 publications below |
产品信息
66814-1-Ig targets CEP250,C-NAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | IHC, IF |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Immunogen | CEP250,C-NAP1 fusion protein Ag5925 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | centrosomal protein 250kDa |
| Synonyms | 250 kDa centrosomal protein, C NAP1, Centrosomal protein 2, centrosomal protein 250kDa, CEP2, CEP250, CEP250,C-NAP1, CNAP1 |
| Calculated Molecular Weight | 281 kDa |
| Observed Molecular Weight | 280 kDa |
| GenBank Accession Number | BC001433 |
| Gene Symbol | CEP250/CNAP1 |
| Gene ID (NCBI) | 11190 |
| RRID | AB_2882157 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BV73 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CEP250, also known as C-Nap1, is a coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP250,C-NAP1 antibody 66814-1-Ig | Download protocol |
| IF protocol for CEP250,C-NAP1 antibody 66814-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience Single-cell proteo-genomic reveals a comprehensive map of centrosome-associated spliceosome components | ||
Pancreatology Altered centriolar cohesion by CEP250 and appendages impact outcome of patients with pancreatic cancer |