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验证数据展示

  • WB analysis using 66821-1-Ig

    WB analysis using 66821-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66821-1-Ig (Syntaxin 10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66821-1-Ig

    IF Staining of HeLa using 66821-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Syntaxin 10 antibody (66821-1-Ig, Clone: 1B1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Syntaxin 10 Monoclonal antibody

Syntaxin 10 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66821-1-Ig

产品说明书

CloneNo. 1B1C3

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

hsyn10, STX10, SYN10, syntaxin 10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, T-47D cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STX10 Ready-To-Use IHC Kit. STX10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66821-1-Ig targets Syntaxin 10 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 Syntaxin 10 fusion protein Ag27861 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 syntaxin 10
别名 hsyn10, STX10, SYN10, syntaxin 10
计算分子量 28 kDa
观测分子量 34 kDa
GenBank蛋白编号BC017237
基因名称 Syntaxin 10
Gene ID (NCBI) 8677
RRIDAB_2882164
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60499
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxin 10 (STX10), also named as SYN10, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes.

实验方案

Product Specific Protocols
WB protocol for Syntaxin 10 antibody 66821-1-IgDownload protocol
IF protocol for Syntaxin 10 antibody 66821-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols