验证数据展示
经过测试的应用
| Positive WB detected in | pig stomach tissue, HT-29 cells |
| Positive IHC detected in | human stomach tissue, mouse pancreas tissue, human colon tissue, human colon cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy ANXA10 Ready-To-Use IHC Kit. ANXA10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66869-1-Ig targets Annexin A10 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, pig, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, pig, mouse |
| 文献引用反应性 | human |
| 免疫原 | Annexin A10 fusion protein Ag27098 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | annexin A10 |
| 别名 | Annexin 10, Annexin 14, annexin A10, ANX14, ANXA10 |
| 计算分子量 | 37 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC007320 |
| 基因名称 | ANXA10 |
| Gene ID (NCBI) | 11199 |
| RRID | AB_2882206 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UJ72 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANXA10, member of the Annexin family, is a calcium-and-phospholipid-binding protein. It is implicated in multiple physiological processes, including growth regulation, cell division, apoptosis, and differentiation. ANXA10 was recently observed to be upregulated in Barrett's esophagus, oral cancers, pancreatic cancer and colon cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Annexin A10 antibody 66869-1-Ig | Download protocol |
| IHC protocol for Annexin A10 antibody 66869-1-Ig | Download protocol |
| IF protocol for Annexin A10 antibody 66869-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
