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验证数据展示

  • WB analysis using 66942-1-Ig

    WB analysis using 66942-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66942-1-Ig (MRRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66942-1-Ig

    IF Staining of HeLa using 66942-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRRF antibody (66942-1-Ig, Clone: 1G5C3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

MRRF Monoclonal antibody

MRRF Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66942-1-Ig

产品说明书

CloneNo. 1G5C3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

RRF, Ribosome-releasing factor, mitochondrial, Ribosome-recycling factor, mitochondrial, mtRRF, MRFF

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, A431 cells, HepG2 cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66942-1-Ig targets MRRF in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MRRF fusion protein Ag28583 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitochondrial ribosome recycling factor
别名 RRF, Ribosome-releasing factor, mitochondrial, Ribosome-recycling factor, mitochondrial, mtRRF, MRFF
计算分子量 262 aa, 29 kDa
观测分子量 25 kDa
GenBank蛋白编号BC013049
基因名称 MRRF
Gene ID (NCBI) 92399
RRIDAB_2882266
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96E11
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRRF antibody 66942-1-IgDownload protocol
IF protocol for MRRF antibody 66942-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols