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PAPPA Monoclonal antibody
PAPPA Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1G5A7
验证数据展示
经过测试的应用
Positive WB detected in | human placenta tissue, MCF-7 cells |
Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:4000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
66962-1-Ig targets PAPPA in WB, IHC, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human |
Cited Reactivity | human |
Immunogen | PAPPA fusion protein Ag11863 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | pregnancy-associated plasma protein A, pappalysin 1 |
Synonyms | ASBABP2, DIPLA1, IGF dependent IGFBP 4 protease, IGFBP 4ase, PAPA, PAPP A, PAPPA, PAPP-A, PAPPA1, Pappalysin 1, Pappalysin-1 |
Calculated Molecular Weight | 1627 aa, 181 kDa |
Observed Molecular Weight | 180-220 kDa |
GenBank Accession Number | BC078657 |
Gene Symbol | PAPPA |
Gene ID (NCBI) | 5069 |
RRID | AB_2882285 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13219 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PAPPA antibody 66962-1-Ig | Download protocol |
IHC protocol for PAPPA antibody 66962-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Oncol Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression. |