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GPR116 Monoclonal antibody

GPR116 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H1B2

Cat no : 66967-2-Ig

Print datasheet

Synonyms

KIAA0758, G-protein coupled receptor 116, G protein coupled receptor 116, Adhesion G protein-coupled receptor F5, ADGRF5

验证数据展示

  • WB analysis using 66967-2-Ig

    WB analysis using 66967-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66967-2-Ig (GPR116 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHUVEC cells, MDA-MB-231 cells, Calu-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66967-2-Ig targets GPR116 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen GPR116 fusion protein Ag5820 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name G protein-coupled receptor 116
Synonyms KIAA0758, G-protein coupled receptor 116, G protein coupled receptor 116, Adhesion G protein-coupled receptor F5, ADGRF5
Calculated Molecular Weight 1346 aa, 149 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC066121
Gene Symbol GPR116
Gene ID (NCBI) 221395
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IZF2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR116 (ADGRF5, adhesion G protein-coupled receptor F5) is involved in the G protein-coupled receptor signaling pathway and cell surface receptor signaling pathway. may act upstream of or within several processes, including glomerular filtration; pharyngeal arch artery morphogenesis; and surfactant homeostasis.

实验方案

Product Specific Protocols
WB protocol for GPR116 antibody 66967-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols